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Hepatic mRNA expression and plasma levels of insulin-like growth factor-I (IGF-I) in broiler chickens selected for different growth rates Genet. Mol. Biol.
Giachetto,Poliana Fernanda; Riedel,Eduardo Correa; Gabriel,Jane Eyre; Ferro,Maria Inês Tiraboschi; Di Mauro,Sônia Marli Zingaretti; Macari,Marcos; Ferro,Jesus Aparecido.
The hepatic expression and plasma concentrations of IGF-I were investigated in three broiler chicken strains selected for different growth rates (HP-Hubbard-Pettersen, a fast growing strain; NN-Naked-neck, a strain with an intermediate growth rate and a heterozygous genotype, and C-Caipira, a slow growing crossbred strain). The chickens were studied at 1, 21 and 42 days of age and had free access to food throughout the study. Hepatic IGF-I mRNA expression was assessed by dot blot analysis using a randomly labeled chicken IGF-I cDNA as the probe and plasma IGF-I concentrations were assayed by radioimmunoassay. The hepatic levels of IGF-I mRNA increased from 1 to 21 days of age in all strains, with NN chickens showing a higher (p < 0.05) IGF-I expression...
Tipo: Info:eu-repo/semantics/article Palavras-chave: IGF-I mRNA; Broiler chicken; Growth performance; Plasma IGF-I.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100007
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Regulation of gene expression in Mycoplasmas: contribution from Mycoplasma hyopneumoniae and Mycoplasma synoviae genome sequences Genet. Mol. Biol.
Madeira,Humberto Maciel França; Gabriel,Jane Eyre.
This report describes the transcription apparatus of Mycoplasma hyopneumoniae (strains J and 7448) and Mycoplasma synoviae, using a comparative genomics approach to summarize the main features related to transcription and control of gene expression in mycoplasmas. Most of the transcription-related genes present in the three strains are well conserved among mycoplasmas. Some unique aspects of transcription in mycoplasmas and the scarcity of regulatory proteins in mycoplasma genomes are discussed.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycoplasma hyopneumoniae; Mycoplasma synoviae; Transcription; Comparative genomics.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200016
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Temporal expression pattern of the insulin-like growth factor II and fibroblast growth factor transcripts in avian embryogenesis BABT
Gabriel,Jane Eyre; Alvares,Lúcia Elvira; Coutinho,Luiz Lehmann.
In this study, the abundance of IGF-II and bFGF transcripts was estimated in the chicken embryos using the competitive RT-PCR analysis. Significant enhancements in the abundance of IGF-II mRNA were observed at stages HH1 and 5, and a new accumulation in these levels was observed at stage HH18 in comparison to the basal levels. The abundance of bFGF mRNA increased significantly at stages HH18 and 20, followed by an upregulation in the expression of these transcripts at stage HH26. These findings provided important information about the temporal expression pattern of IGF-II and bFGF transcripts in the whole chicken embryos during in ovo development.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chicken embryos; Temporal expression; Competitive RT-PCR; Growth factors.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500011
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